Journal: Advanced Science

Article Title: Activation of USP30 Disrupts Endothelial Cell Function and Aggravates Acute Lung Injury Through Regulating the S‐Adenosylmethionine Cycle

doi: 10.1002/advs.202512807

Figure Lengend Snippet: Depletion of USP30 in ECs ameliorates endotoxin‐induced acute lung injury. A) Scheme shows the targeting strategy for generating the EC‐USP30KO mice. B) Immunoblotting analysis of USP30 from isolated lung ECs from Wt and EC‐USP30KO mice. C) USP30 expression was deleted in CD31‐positive cells in EC‐USP30KO mouse lungs, compared to wild‐type (Wt) mice. IgG was used as a negative control. Br, Bronchus. D) Isolated lung ECs (from Wt and EC‐USP30KO mice) were cultured on ECIS chambers to confluence. TEER was measured for cell permeability. Data shown as mean ± SEM, n = 3. E–H) Mice were challenged with (i.t.) LPS (5 mg kg −1 body weight) for 24 h. Protein levels (E), IL‐6 (F), IL‐1β (G), and neutrophil influx (H) in BAL were measured. Data shown as mean ± SEM, n = 6. I) H&E staining of lung tissues. Scale bar, 50 µm. J) Evans Blue in vivo leakage assay after (i.t.) LPS. K) Lung wet/dry ratio was measured. L) Mice were challenged with (i.p.) LPS (2 mg kg −1 body weight) for 24 h. Evans Blue in vivo leakage assay after (i.p.) LPS. Data shown as mean ± SEM, n = 6. M,N) IL‐1β and IL‐6 in BAL were measured by ELISA. Data shown as mean ± SEM, n = 6. O,P) IL‐1β and IL‐6 in plasma were measured by ELISA. Data shown as mean ± SEM, n = 6.

Article Snippet: GAPDH , Mouse / IgG2 , 1:2000 , Proteintech, 60004‐1‐Ig.

Techniques: Western Blot, Isolation, Expressing, Negative Control, Cell Culture, Permeability, Staining, In Vivo, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

Journal: Frontiers in Immunology

Article Title: Larval ascariasis elicits a prominent IgA and IgG1/2 antibody response to adult Ascaris excretory/secretory antigens in pigs

doi: 10.3389/fimmu.2025.1606128

Figure Lengend Snippet: Antibody isotype responses against A . suum L3, adult and adult ES antigens in serum and bile 35 dpi. (A) Schematic illustrating experimental infection of the pigs. Eight pigs were infected with a single dose of 4000 infective A . suum eggs. In parallel, four uninfected controls were included. Box plots illustrating median serum (B) IgM, IgG, IgA and (C) IgG1 and IgG2 subclass responses against A . suum L3 lysate, adult lysate and adult ES products. (D) Box plots illustrating median bile IgM, IgG and IgA responses against A . suum L3 lysate, adult lysate and adult ES products. The data is reported in arbitrary ELISA units (AEU). Whiskers indicate 95% percentile. Significance assessed using the Wilcoxon test and depicted by p<0.05: *p<0.01: **p<0.001: ***p<0.0001: ****. Ascaris -infected n= 16 and uninfected controls n= 8.

Article Snippet: For IgM, IgA, IgG1 and IgG2, non-conjugated mouse anti-pig IgM (Bio-Rad, # MCA637GA) diluted at 1:20000, mouse anti-pig IgA (Bio-Rad, # MCA638GA) diluted at 1:1000, mouse anti-pig IgG1 (Bio-Rad, # MCA635GA) diluted at 1:1000 and mouse anti-pig IgG2 (Bio-Rad, # MCA638GA) diluted at 1:1000, mouse anti-pig sIgA (Bio-Rad, # MCA634GA) diluted at 1:10000 was used.

Techniques: Infection, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Larval ascariasis elicits a prominent IgA and IgG1/2 antibody response to adult Ascaris excretory/secretory antigens in pigs

doi: 10.3389/fimmu.2025.1606128

Figure Lengend Snippet: Comparable Ascaris -specific antibody responses in Ascaris single- and co-infected pigs. (A) Schematic illustrating experimental infection of the pigs. Box plots illustrating median serum IgM, IgG, IgA, IgG1 and IgG2 responses (B) and median bile IgM, IgG and IgA responses (C) against A . suum adult ES products. (D) Box plots illustrating L3- and adult-specific IgA responses in bile and serum. Blue, red, orange and green represent uninfected controls, A. suum infected, A . suum- S . Typhimurium coinfected and S. Typhimurium infected pigs respectively. Whiskers indicate 95% percentile. Significance is represented by p<0.05: *p<0.01: **p<0.001: ***p<0.0001: ****. Significance was tested using Kruskal Wallis with Dunn or Games-Howell test and Wilcoxon test. Uninfected controls n = 12, Ascaris single infected n = 12, Ascaris-Salmonella coinfected n = 12, Salmonella single infected n =12.

Article Snippet: For IgM, IgA, IgG1 and IgG2, non-conjugated mouse anti-pig IgM (Bio-Rad, # MCA637GA) diluted at 1:20000, mouse anti-pig IgA (Bio-Rad, # MCA638GA) diluted at 1:1000, mouse anti-pig IgG1 (Bio-Rad, # MCA635GA) diluted at 1:1000 and mouse anti-pig IgG2 (Bio-Rad, # MCA638GA) diluted at 1:1000, mouse anti-pig sIgA (Bio-Rad, # MCA634GA) diluted at 1:10000 was used.

Techniques: Infection

Journal: Frontiers in Immunology

Article Title: Larval ascariasis elicits a prominent IgA and IgG1/2 antibody response to adult Ascaris excretory/secretory antigens in pigs

doi: 10.3389/fimmu.2025.1606128

Figure Lengend Snippet: Strong positive correlation between sIgA against A . suum adult ES and eosinophil influx. (A) Pearson correlation of sIgA against adult ES products and %eosinophil frequencies of leukocytes in BAL . (B) Box plots illustrating median IgM, IgG and IgA responses against A . suum adult ES products in BAL fluid. (C) Box plots illustrating sIgA against L3 lysate, adult lysate and adult ES in BAL fluid. Blue and red denotes uninfected controls and A. suum infected pigs, respectively. Whiskers indicate 95% percentile. Significance determined by Wilcoxon test is represented by p<0.05: *p<0.01: **.

Article Snippet: For IgM, IgA, IgG1 and IgG2, non-conjugated mouse anti-pig IgM (Bio-Rad, # MCA637GA) diluted at 1:20000, mouse anti-pig IgA (Bio-Rad, # MCA638GA) diluted at 1:1000, mouse anti-pig IgG1 (Bio-Rad, # MCA635GA) diluted at 1:1000 and mouse anti-pig IgG2 (Bio-Rad, # MCA638GA) diluted at 1:1000, mouse anti-pig sIgA (Bio-Rad, # MCA634GA) diluted at 1:10000 was used.

Techniques: Infection

Journal: Parasite Immunology

Article Title: Allotype‐Dependent Responses to the Vaccine Candidate Thrombospondin‐Like Protein of Dictyocaulus viviparus in Calves

doi: 10.1111/pim.70013

Figure Lengend Snippet: IgG2 responses measured with mab of the vaccinated calves (left column) and the control calves (right column) against TLP (upper panel), TLP protein moiety (middle panel) and TLP glycan moiety (TLP minus TLP protein, lower panel). Filled arrow heads indicate time of vaccination and open arrow heads indicate day of challenge infection. OD correlates with worm counts * p < 0.05, ** p < 0.005.

Article Snippet: Total IgG2 ELISA with the monoclonal antibody was performed with the Bovine IgG2 ELISA quantification kit (Bethyl laboratories.inc.), but instead of the polyclonal conjugate, mouse anti‐IgG2 specific monoclonal antibody (Bio‐Rad, mca 626) was used (1:2000), followed by a 1:2000 goat antimouse Ig/AP (DAKO cat nr. D0486).

Techniques: Control, Glycoproteomics, Infection

Journal: Parasite Immunology

Article Title: Allotype‐Dependent Responses to the Vaccine Candidate Thrombospondin‐Like Protein of Dictyocaulus viviparus in Calves

doi: 10.1111/pim.70013

Figure Lengend Snippet: Total IgG2 in serum of Day 0, 28 and 70 from vaccinated (top) and control (bottom) calves. Measured with mab directed against bovine IgG2.

Article Snippet: Total IgG2 ELISA with the monoclonal antibody was performed with the Bovine IgG2 ELISA quantification kit (Bethyl laboratories.inc.), but instead of the polyclonal conjugate, mouse anti‐IgG2 specific monoclonal antibody (Bio‐Rad, mca 626) was used (1:2000), followed by a 1:2000 goat antimouse Ig/AP (DAKO cat nr. D0486).

Techniques: Control